{"id":669,"date":"2023-03-15T13:12:52","date_gmt":"2023-03-15T06:12:52","guid":{"rendered":"https:\/\/conf.icgbio.ru\/bgrs98\/?page_id=669"},"modified":"2023-04-11T14:31:23","modified_gmt":"2023-04-11T07:31:23","slug":"051_functional-activity-of-a-novel-gcc-element-found-in-homologous-regulatory-regions-of-some-human-genes","status":"publish","type":"page","link":"https:\/\/conf.icgbio.ru\/bgrs98\/abstracts\/abstract-list\/051_functional-activity-of-a-novel-gcc-element-found-in-homologous-regulatory-regions-of-some-human-genes\/","title":{"rendered":"FUNCTIONAL ACTIVITY OF A NOVEL GCC-ELEMENT FOUND IN HOMOLOGOUS REGULATORY REGIONS OF SOME HUMAN GENES"},"content":{"rendered":"<p><a href=\"https:\/\/conf.icgbio.ru\/bgrs98\/abstracts\/authors-index\/#perevozchikov\">PEREVOZCHIKOV A.<\/a>,\u00a0<a href=\"https:\/\/conf.icgbio.ru\/bgrs98\/abstracts\/authors-index\/#orlov\">ORLOV S.<\/a><\/p>\n<p>Institute of Experimental Medicine, Rus. Acad. Med. Sci.<\/p>\n<p><a href=\"https:\/\/conf.icgbio.ru\/bgrs98\/abstracts\/keywords-index\/\">Keywords<\/a>: functional activity, GCC element, regulatory regions, GC-rich regions<\/p>\n<p>&nbsp;<\/p>\n<p>The homologous GC-rich regions were found in regulatory areas of some mammalian (including human)genes by computer-assisted analysis. The core components of these regions are triplet repeats (GCC)n (n=3-30), which may be considered as a putative cis-acting element (GCC-element). On studying of the murine ribosomal protein L32 (rpL32) promoter that contains GC(GCC)4 sequence, we have revealed this GCC-element can bind human (HeLa, fibroblasts) and murine (NIH 3T3) nuclear proteins in gel-retardation experiments. Dependence of these interactions on zinc ions was also showed. The molecular weight of GCC-element binding proteins from HeLa and human embryo cells determined by South-Western assay were 18-20 kDa and 28-31 kDa respectively. The GCC-element binding proteins were purified by DNA-affinity chromatography from 4 &#8211; 5 week human embryos and partially characterized.<\/p>\n<p>Cat-assay (NIH 3T3 cells) demonstrated that GCC-element acts in the rpL32 promoter as a positive cis-acting element. However, transfer of this element to minimal HSV-1 timidine kinase (TK) promoter leads to its repression. The nearest regulatory sequence to GCC-element is well known Egr-element (1 bp difference). However, Egr1 transcription factor did not affect activity of rpL32 promoter in co-transfection experiments that agrees with incapacity of Egr family transcription factors to bind rpL32 promoter in vitro. At the same time, Egr1 partially abolished GCC-element-dependent repression of TK promoter. Thus, we are describing here the novel cis-acting GC-rich element, which may be important for coordinated regulation of expression of some mammalian (human) genes.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PEREVOZCHIKOV A.,\u00a0ORLOV S. Institute of Experimental Medicine, Rus. Acad. Med. Sci. Keywords: functional activity, GCC element, regulatory regions, GC-rich regions &nbsp; The homologous GC-rich regions were found in regulatory areas of some mammalian (including human)genes by computer-assisted analysis. The core &hellip; <a href=\"https:\/\/conf.icgbio.ru\/bgrs98\/abstracts\/abstract-list\/051_functional-activity-of-a-novel-gcc-element-found-in-homologous-regulatory-regions-of-some-human-genes\/\">Continue reading <span class=\"meta-nav\">&rarr;<\/span><\/a><\/p>\n","protected":false},"author":13,"featured_media":0,"parent":97,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/conf.icgbio.ru\/bgrs98\/wp-json\/wp\/v2\/pages\/669"}],"collection":[{"href":"https:\/\/conf.icgbio.ru\/bgrs98\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/conf.icgbio.ru\/bgrs98\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/conf.icgbio.ru\/bgrs98\/wp-json\/wp\/v2\/users\/13"}],"replies":[{"embeddable":true,"href":"https:\/\/conf.icgbio.ru\/bgrs98\/wp-json\/wp\/v2\/comments?post=669"}],"version-history":[{"count":3,"href":"https:\/\/conf.icgbio.ru\/bgrs98\/wp-json\/wp\/v2\/pages\/669\/revisions"}],"predecessor-version":[{"id":1388,"href":"https:\/\/conf.icgbio.ru\/bgrs98\/wp-json\/wp\/v2\/pages\/669\/revisions\/1388"}],"up":[{"embeddable":true,"href":"https:\/\/conf.icgbio.ru\/bgrs98\/wp-json\/wp\/v2\/pages\/97"}],"wp:attachment":[{"href":"https:\/\/conf.icgbio.ru\/bgrs98\/wp-json\/wp\/v2\/media?parent=669"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}