Changes in the size of cell nuclei in response to SMC proteins degradation in mammalian cells

Myakinkov Ivan Olegovich; Khabarova Anna Aleksandrovna; Yunusova Anastasia Maratovna; Battulin Nariman Rashitovich

Novosibirsk State University, Novosibirsk, Russia; Institute of Cytology and Genetics, SB RAS, Novosibirsk, Russia

SMC complexes condensin and cohesin are essential for the three-dimensional organization of genome and cell division. However, a lot about their function remains unknown. We apply a proteasomal degradation approach to these complexes in order to examine the knockout phenotype in cell cultures. During these experiments, we have noticed a notable enlargement of the cell nuclei. We decided to inspect this phenomenon.