Kriukova K.I.; Gancheva M.S.; Lutova L.A.
Saint Petersburg State University, Universitetskaya nab., 7–9, Saint Petersburg, Russia; All-Russia Research Institute for Agricultural Microbiology, Shosse Podbel’skogo, 3, Saint Petersburg, Russia
Auxin is a key regulator of plant growth and development, and the synthetic auxin-sensitive DR5 promoter is often used for study auxin distribution. Several tuber-forming plants were selected to analyze the activity of the DR5 promoter: Oxalis tuberosa Mol., Stachys sieboldii Mig., Apios americana Medik, Cyperus esculentus L., Solanum phureja Juz. et Buk. Different methods for sterilization of explants were tested. We also began to work out the agrobacterial transformation of plants. We have successfully introduced all of the above plants into in vitro culture. Transgenic roots of Oxalis tuberosa, Stachys sieboldii, and Solanum phureja were obtained — the activity of the DR5 promoter in these plants was found at the root tip.
