Kolosovskaya E.V.*1,2; Gerasimova S.V.1,2; Korotkova A.M.1; Hertig С.3; Morozov S.V.4; Chernyak E.I.4; Domrachev D.V.4; Vikhorev A.В.1,2; Shmakov N.A.1; Kochetov A.V.1,2; Kumlehn J.3; Khlestkina E.K.1,2,5
1Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia; 2Novosibirsk State University, Novosibirsk, Russia; 3Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany; 4Novosibirsk Institute of Organic Chemistry, SB RAS, Novosibirsk, Russia; 5Vavilov Institute of Plant Genetic Resources, Saint Petersburg, Russia
Isogenic lines with contrasting phenotypes are a perfect experimental model system for the elucidation of gene functions. The Cas9/gRNA-mediated knockout of the barley (Hordeum vulgare) WAX INDUCER 1 (HvWIN1) gene, that encodes an APETALA2/ETHYLENE-RESPONSIVE FACTOR, resulted in epicuticular wax deficiency and glossy sheath phenotype in primary mutants of cv. ‘Golden Promise’ and their progeny. Six homozygous lines with two different mutations in the WIN1 gene were established. Сomprehensive phenotyping, scanning electron microscopy, wax measurement, and comparative transcriptome analysis were performed. Based on this data, it is concluded that the WIN1 transcription factor is a major and specific trigger of wax biosynthesis in leaf sheaths during generative development in barley.
PlantGen-2021_KolosovskayaEV
