Advanced panel of molecular markers identifying of stem rust resistance genes Sr2, Sr15, Sr22, Sr23, Sr24, Sr25, Sr26, Sr31, Sr35, Sr36, Sr38, Sr39, Sr44, Sr45, Sr57, Lr6Ai#2 in Siberian wheat cultivars

Kelbin V.N., Skolotneva E.S.; Shamanin V.P.; Salina E.A.
Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia; Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia; Omsk State Agrarian University named after P.A. Stolypin, Omsk, Russia; Institute of Cytology and Genetics SB RAS, Novosibirsk, Russia
Wheat stem rust caused by the fungus Puccinia graminis f. sp. tritici is a dangerous disease that has destructed the wheat crops around the world for centuries. The most effective, economical and environmentally friendly way to protect wheat from stem rust outcomes is to cultivate resistant varieties of wheat. Before this, germplasm and wild relatives assessment is required to identify resistance genes that are effective against both the local pathogen and the Ug99 race. The aim of this study was to identify genes and gene loci for resistance to wheat stem rust in Russian wheat germplasm using advanced panel of markers. The germplasm for analysis included 224 varieties and lines of wheat, the collections of which were kindly provided by: (1) FSBEI HE Omsk SAU (Omsk region) – 80 varieties / lines of spring soft wheat and 12 lines of spelt wheat; (2) FRC IC&G SB RAS (Novosibirsk region), consisting of 132 varieties of spring soft wheat. Identification of resistance genes were carried out using the DNA-markers recommended for marker selection (MAS).